重奖【0001号】基金论文版活动

lomson

Membrane Shape at the Edge of the Dynamin Helix Sets Location and Duration of the Fission Reaction

SUMMARY
The GTPase dynamin polymerizes into a helical coat that constricts membrane necks of endocytic pits to promote their fission. However, the dynamin mechanism is still debated because constriction is necessary but not sufficient for fission. Here, we show that fission occurs at the interface between the dynamin coat and the uncoated membrane. At this location, the considerable change in membrane curvature increases the local membrane elastic energy, reducing the energy barrier for fission. Fission kinetics depends on tension, bending rigidity, and the dynamin constriction torque. Indeed, we experimentally find that the fission rate depends on membrane tension in vitro and during endocytosis in vivo. By estimating the energy barrier from the increased elastic energy at the edge of dynamin and measuring the dynamin torque, we show that the mechanical energy spent on dynamin constriction can reduce the energy barrier for fission sufficiently to promote spontaneous fission.

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文献全文参考附件

lomson

wangbingxhy 发表于 2012-11-2 00:19
动力蛋白螺旋设置位点附近和裂变反应期间的膜形状研究
概要
GTP酶动力蛋白聚合成一个螺旋形的外套来压缩膜 ...

谢谢您的参与，翻译的很好，底子不错，学习了。很受益

wangbingxhy

动力蛋白螺旋设置位点附近和裂变反应期间的膜形状研究
概要
GTP酶动力蛋白聚合成一个螺旋形的外套来压缩膜颈部上的内吞坑，以促进他们的裂变。然而，因为对于裂变，压缩仅是必要条件而非充分条件，所以，其动力机制未明。我们观察到裂变发生在动力蛋白外套膜和未包被膜相交处。在此位置，膜的曲率通过发生明显的改变来增加了局部的膜弹性能量，从而减少裂变的能量壁垒。裂变动力学取决于膜张力，弯曲刚度和动力收缩矩。事实上，通过实验发现，裂变率取决于在外的膜张力和膜的内吞过程。通过评估动力蛋白边缘的弹性能量的增加引起的能量壁垒改变和测量动力钜，我们揭示出，花费在动力蛋白收缩上的机械能可以足以减少裂变能量壁垒到使裂变自发产生。



翻的太乱了，自己都不知道怎么回事，本不想献丑的，版主邀请了，就捧个场吧，实在太菜了，重在参与吧。

lomson

怎么没有人响应呀，
鼓励大家在本版发布自己本专业或领域的论文作为活动内容，一块学习

lomson

没有人参与呀。我先来吧，有这方面专业的会员多给点建议，毕竟我也不是专业的。

Membrane Shape at the Edge of the Dynamin Helix Sets Location and Duration of the Fission Reaction

SUMMARY
The GTPase dynamin polymerizes into a helical coat that constricts membrane necks of endocytic pits to promote their fission. However, the dynamin mechanism is still debated because constriction is necessary but not sufficient for fission. Here, we show that fission occurs at the interface between the dynamin coat and the uncoated membrane. At this location, the considerable change in membrane curvature increases the local membrane elastic energy, reducing the energy barrier for fission. Fission kinetics depends on tension, bending rigidity, and the dynamin constriction torque. Indeed, we experimentally find that the fission rate depends on membrane tension in vitro and during endocytosis in vivo. By estimating the energy barrier from the increased elastic energy at the edge of dynamin and measuring the dynamin torque, we show that the mechanical energy spent on dynamin constriction can reduce the energy barrier for fission sufficiently to promote spontaneous fission.

螺旋状发动蛋白聚集点附近细胞膜形状和膜裂变反应研究
摘要
GTP酶发动蛋白聚合成螺旋状包被在膜表面，从而促使细胞膜收缩形成内吞环而发生裂变。然而，在此过程中发动蛋白的作用机制仍存争议。因为细胞膜收缩并不足以引起裂变。本文我们研究了发动蛋白包被和未包被细胞膜交界处裂变发生情况。两者的交界处，膜曲率发生大的改变，使膜弹性能量增加，从而降低膜裂变能量势垒。裂变动力学取决于拉伸，抗弯刚度和动力蛋白收缩扭矩。我们通过实验确实发现，细胞膜裂变的速度取决在膜外张力和膜内吞作用。通过发动蛋白边缘膜弹性能量增加量评估能量势垒和发动蛋白扭矩度检测，我们发现用于发动蛋白收缩的能量能减少细胞膜裂变所需能量势垒，促进细胞膜自发裂变。

lomson

eagle360 发表于 2012-10-30 15:44
翻译是技术活  你那奖励BB  不远远不够的

重在参与，可以再提高

eagle360

翻译是技术活  你那奖励BB  不远远不够的

lomson

顶一下，快来参加吧