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速递书局
| 封面: |
 |
| 题名: |
Basic Cell Culture Protocols |
| 作者: |
Cheryl D. Helgason，Cindy L. Miller |
| 出版社: |
humma press |
| 出版日期: |
2005 |
| ISBN: |
978-1-62703-127-1 |
| 附属页: |
齐全 |
| 书签: |
无 |
| 格式: |
清晰PDF |
| 内容简介: |
Cell culture is an invaluable tool for investigators in numerous fields. It facilitates
analysis of biological properties and processes that are not readily accessible at the level
of the intact organism. Successful maintenance of cells in culture, whether primary or
immortalized, requires knowledge and practice of a few essential techniques. The purpose
of this chapter is to explain the basic principles of cell culture using the maintenance
of a nonadherent cell line, the P815 mouse mastocytoma cell line, and the isolation
and culture of adherent primary mouse embryonic fibroblasts (MEFs) as examples.
Procedures for thawing, culture, determination of cell numbers and viability, and
cryopreservation are described. |
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发表于 2013-3-11 08:52:09
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