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标题: High-efficiency yeast transformation using the LiAc/SS carrier DNA/PEG method [打印本页]

作者: world663 时间: 2015-1-31 10:44
标题: High-efficiency yeast transformation using the LiAc/SS carrier DNA/PEG method

题名	High-efficiency yeast transformation using the LiAc/SS carrier DNA/PEG method
链接	http://www.nature.com/nprot/journal/v2/n1/full/nprot.2007.13.html

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igh-efficiency yeast transformation using the LiAc/SS carrier DNA/PEG method

R Daniel Gietz1 & Robert H Schiestl2

Abstract
Here we describe a high-efficiency version of the lithium acetate/single-stranded carrier DNA/PEG method of transformation of Saccharomyces cerevisiae. This method currently gives the highest efficiency and yield of transformants, although a faster protocol is available for small number of transformations. The procedure takes up to 1.5 h, depending on the length of heat shock, once the yeast culture has been grown. This method is useful for most transformation requirements.

http://www.nature.com/nprot/journal/v2/n1/full/nprot.2007.13.html

作者: vip 时间: 2015-1-31 10:44
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作者: vip 时间: 2015-1-31 10:44
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