Peripheral primitive neuroectodermal tumor of the ovary confirmed by CD99 imm... - 科研速递论坛

题名	Peripheral primitive neuroectodermal tumor of the ovary confirmed by CD99 immunostaining, karyotypic analysis, and RT-PCR for EWS/FLI-1 chimeric mRNA.
作者	Kawauchi S, Fukuda T, Miyamoto S, Yoshioka J, Shirahama S, Saito T, Tsukamoto N.
杂志	Am J Surg Pathol.
年\|卷\|期	1998 Nov;22(11):1417-22.
页码	1998 Nov;22(11):1417-22.
链接	https://www.ncbi.nlm.nih.gov/pubmed/?term=Peripheral+primitive+neuroectodermal+tumor+of+the+ovary+confirmed+by+CD99+immunostaining%2C+karyotypic+analysis%2C+and+RT-PCR+for+EWS%2FFLI-1+chimeric+mRNA.

Am J Surg Pathol. 1998 Nov;22(11):1417-22.

Peripheral primitive neuroectodermal tumor of the ovary confirmed by CD99 immunostaining, karyotypic analysis, and RT-PCR for EWS/FLI-1 chimeric mRNA.

Kawauchi S, Fukuda T, Miyamoto S, Yoshioka J, Shirahama S, Saito T, Tsukamoto N.

Abstract
We report a case of peripheral primitive neuroectodermal tumor (pPNET), which belongs to the PNET/Ewing's sarcoma family, arising in the left ovary of a 29-year-old woman. Microscopically, the tumor was composed of solid nests and sheets of monotonous, primitive, small round cells with a few rosettes, making it difficult to distinguish from small cell carcinoma of the ovary. Immunohistochemically, the tumor cells showed intense cell-membranous immunoreactivity for MIC2 protein (CD99). A short-term cell culture and karyotypic analysis revealed the tumor to possess a balanced t(11;22)(q24;q12) chromosomal translocation that is highly specific for tumors of the PNET/Ewing's sarcoma family. In addition, EWS/FLI-1 chimeric mRNA that originated from the characteristic chromosomal translocation was detected by reverse transcription-polymerase chain reaction. These results confirmed the diagnostic validity of the present tumor being a pPNET, thus raising the possibility that in the past, pPNETs which have arisen in the ovary may have been mistakenly diagnosed as small cell carcinomas of the ovary.

PMID: 9808135